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KMID : 1132720040020030113
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Genomics & Informatics
2004 Volume.2 No. 3 p.113 ~ p.120
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Current Status and Future Clinical Applications of Array?based Comparative Genomic Hybridization
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Yim Seon-Hee
Chung Yeun-Jun
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Abstract
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Constitutional chromosomal alterations are commonly detected features in various human diseases such as tumor, congenital anomalies, psychiatric disorders, and metabolic disorders. Neoplastic transformations, for example, are initiated by the aberrations of genes regulating cell proliferation, apoptosis, genome stability, angiogenesis, invasion and metastasis (Hanahan and Weinberg, 2000). Through the population genetics studies, some tumor suppresor genes and oncogenes have been verified (Wakabayashi et al., 2003; Huges et al., 2001; Wilentz et al., 2001; Herranz et al., 1999). And the causative chromosomal alterations for some congenital genetic disorders have been identified by conventional cytogenetic tools. However, there will be even more unknown tumor-related genes, supposedly up to several hundreds,
yet to be found (Balmain, 2002), and still a lot of idiopathic psychiatric/ metabolic disorders of unknown origin. In this aspect, precise detection of the breakpoint of chromosomal dosage change, together with the functional and clinical studies, is essential to understand the causes of these disorders and to prevent them. Microarray technology makes it possible to do highthroughput and high-resolution analysis. Combination of conventional comparative genomic hybridization (CGH) and microarray technology promises us genome-wide high-resolution DNA copy number analysis. We review here the recent progress of the array based CGH (A-CGH) technology and its clinical applications.
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KEYWORD
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array-comparative genomic hybridization, chromosome aberration, BAC, cancer, congenital disease
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